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Viagen Biotech Inc.是創(chuàng)新的DNA提取技術(shù)和生命科學(xué)研究產(chǎn)品的供應(yīng)商。ViagenDirectPCR®DNA提取系統(tǒng)是一種單管系統(tǒng)，用于從小鼠尾巴，耳朵片，卵黃囊和培養(yǎng)細(xì)胞中快速制備DNA。由Viagen Biotech Inc.的科學(xué)家開發(fā)的正在申請(qǐng)的組件允許得到的DNA提取物與用于基因分型的基因組PCR相容。生物樣品的粗提取物與許多分子生物學(xué)級(jí)反應(yīng)（如聚合酶鏈反應(yīng)（PCR））不相容，部分原因是粗提物中含有抑制劑。DirectPCR試劑不僅介導(dǎo)生物樣品的快速溶解，而且還含有有效抑制粗裂解物對(duì)PCR擴(kuò)增的抑制活性的抑制劑，同時(shí)zui大限度地保持了釋放的基因組DNA的完整性。該過程*消除了任何溶液轉(zhuǎn)移或開管步驟，節(jié)省了大量的時(shí)間和精力。DirectPCR試劑每尾只花費(fèi)26美分，可以節(jié)省任何使用量。

Viagenbiotech致力于簡便快速提取和制備核酸（DNA / RNA），以批量、快速進(jìn)行基因分析。公司擁有的技術(shù)不僅加快樣品的裂解，而且降低了粗提物中PCR抑制因素，因而使DNA提取與PCR基因分析兼容，樣品經(jīng)一步消化后即可進(jìn)行PCR擴(kuò)增，并且保證了基因組的完整性。

DirectPCR Lysis Reagent (Mouse Tail) 100ml

快速概述

目錄號(hào)：102-T

zui多500尾（100ml）

在4℃下穩(wěn)定長達(dá)12個(gè)月 

描述

Viagen DirectPCR® DNA Extraction System is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells. The patent-pending components developed by scientists at Viagen Biotech Inc. allow the resulting DNA extracts to be compatible with genomic PCR for genotyping. Crude extracts of biological samples are not compatible with many molecular biology-grade reactions such as polymerase chain reaction (PCR), in part due to inhibitors contained in crude extracts. The DirectPCR reagents not only mediate the rapid lysis of biological samples but also contain inhibitors that effectively suppress the inhibitory activities of crude lysates for PCR amplification, while maximally maintaining the integrity of released genomic DNA. The patent-pending simple procedure compley eliminate any solution transfer or tube-opening steps, providing you with substancial extra time.

Brief procedure
1.  Lyse tails in DirectPCR® Lysis Reagent.
2.  Incubate for 45 min at 85°C.
3.  PCR genotyping with 1 μl lysates.

Detailed protocols:  Tail, Ear, Yolk Sac, and Cultured cells.

DirectPCR® system offers advantages over conventional protocols that include:
· Time saving: Less hands-on time. Crude tail lysates for PCR.
· Money saving: Cost-effective reactions.
· Safe: No organic reagents. 
· Environmental: Less waste (organic reagents, tubes, tips, etc...) 
· Reliable and efficient: Virtually 100% success rate with high yields.

DirectPCR Lysis Reagent (mouse tail)

101-T 50ml (250 mouse tails).

102-T 100ml (500 mouse tails).